jawsii mouse dendritic cell line  (ATCC)

Journal: Viruses

Article Title: HSV-1 Infection of Epithelial Dendritic Cells Is a Critical Strategy for Interfering with Antiviral Immunity

doi: 10.3390/v14051046

Figure Lengend Snippet: HSV-1 enters dendritic cells via the binding of gD protein to the HVEM receptor and replicates in the cells. The untreated JAWSII dendritic cells were deemed the experimental group, and the two groups of JAWSII dendritic cells treated with anti-gD specific antibody or anti-HVEM specific antibody were deemed the antibody blocking group. ( A ) Growth curves for the McKrae strains from the experimental and antibody blocking groups. ( B ) mRNA expression levels of genes and structural proteins such as ICP0 and UL41 of the McKrae strains in the experimental and antibody blocking groups. ( C ) Transcript levels of the IFN family, GM-CSF, TNF-α, TGF-β, IL-4, and IL-6 in the experimental and antibody blocking groups. ( D ) Expression of the maturation markers CD83 and MHC-I on the dendritic cell surface in the experimental and antibody blocking groups. The relative expression levels of inflammatory cytokines in JAWSII dendritic cells were normalized to their levels in the blank control group by using the comparative Ct (ΔΔCt) method. The data are from three independent experiments that were run in duplicate. Statistical significance was assessed by two-way ANOVA with Holm–Sidak adjustment for multiple comparisons (*, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001).

Article Snippet: The African green monkey kidney cell line Vero (ATCC, Rockefeller, MD, USA) and mouse marrow immature dendritic cell line JAWSII (ATCC, Rockefeller, MD, USA) were purchased and maintained in the viral immunization room of IMB, CAMS.

Techniques: Binding Assay, Blocking Assay, Expressing, Control